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CST - Phospho-PKR Thr451 Antibody.

Rabbit polyclonal to PkR Phospho-Thr446. PKR a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of. Phospho-PKR Thr446 Antibody, CatCAF10987 - Biomatik Biomatik provides products of ELISA Kits, Antibodies, Proteins, Biochemicals & Enzymes. Also, we deal with the services of Gene Synthesis, Peptide Synthesis, Protein Expression at affordable charges. Anti-phospho-PKR Thr446 Antibody is an antibody against phospho-PKR Thr446 for use in WB. eCl@ss 32160702 NACRES NA.41 Certificate of Analysis COA.

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Specificity: Phospho-PKR Thr446 Antibody detects endogenous levels of PKR only when phosphorylated at Threonine 446. Anti-phospho-PKR Thr446 Antibody is an antibody against phospho-PKR Thr446 for use in WB. - Find MSDS or SDS, a COA, data sheets and more information. It appears that your browser has JavaScript disabled. Western blot analysis of extracts from HeLa cells, untreated or IFN-alpha/beta-treated 1000 U/ml for 18 hours, and subsequently untreated or calyculin A-treated 0.1 µM for 15 minutes, using Phospho-PKR Thr451 Antibody upper or PKR Antibody 3072 lower. Phosphorylation of Thr446 and Thr451 in the PKR activation loop is required in vivo and in vitro for high level kinase activity. PKR phosphorylates its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor 2 EIF2 alpha, leading to the inhibition of protein synthesis. Immunohistochemistry-Paraffin: PKR [p Thr446] Antibody SY230 [NBP2-67426] - Analysis of paraffin-embedded human kidney tissue using anti-Phospho-PKRT446 antibody.

The antiserum was produced against synthesized peptide derived from human PKR around the phosphorylation site of Thr446. Immunogen Range: 413-462 Physical form Rabbit IgG in phosphate buffered saline without Mg2 and Ca2, pH 7.4, 150mM. PKR Thr446Thr451 Antibody Formalin-fixed and paraffin embedded rat brain labeled with Anti Phospho-PKR Thr446Thr451 Polyclonal Antibody bs-3337R, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining. Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF treated 100 ng/ml, using Phospho-Akt Thr308 Antibody upper or Akt Antibody 9272 lower. This product has been approved for use in this application by CST.

Phospho-PKR Thr446 Rabbit anti-Human, Polyclonal, MilliporeSigma 100µg; Unlabeled Life Sciences:Antibodies:Primary Antibodies:Western Blotting WB. Phospho-PKR Thr446 Polyclonal Antibody Catalog Number PA5-37704 Product Data Sheet Product Specific Information A suggested positive control for Western blot is K562 cells; suggested positive control for IHC is human colon carcinoma; suggested positive control for ICC/IF is Hela cells. Background/Target Information.PKR is a 68 kDa protein and an IFN-induced serine/threonine protein kinase consisting of two N-terminal RNA-binding regulatory domains and a functional C-terminal serine/threonine protein kinase domain. Activated by dsRNA produced during viral infections, PKR plays a key role in IFN.

PKR antibody LS-C17480 is an unconjugated rabbit polyclonal antibody to human PKR EIF2AK2. Validated for WB. More>> This Anti-phospho-PKR Thr451 Antibody is validated for use in WB for the detection of phospho-PKR Thr451. Less<< Anti-phospho-PKR Thr451 Antibody MSDS material safety data sheet or SDS, CoA and CoQ, dossiers, brochures and other available documents. Anti-phospho-PKR Thr446 Antibody.

II. Primary Antibody Incubation. Incubate membrane and primary antibody at the appropriate dilution and diluent as recommended in the product datasheet in 10 ml primary antibody dilution buffer with gentle agitation overnight at 4°C. Wash three times for 5 min each with 15 ml of TBST. Phospho-PKR Thr451 Western blot analysis of extracts from HeLa cells, untreated or INF-a/b-treated 1000 U/ml for 18 hours, and subsequently untreated or calyculin A-treated 0.1 µM for 15 minutes, using Phospho-PKR Thr451 Antibody upper or PKR Antibody 3072 lower. Specificity / Sensitivity. Phospho-AMPKalpha Thr172 Antibody detects endogenous AMPKα only when phosphorylated at threonine 172. The antibody detects both α1 and α2 isoforms of the catalytic subunit, but it does not detect the regulatory beta or gamma subunits. The precipitated proteins were analyzed as indicated by SDS gel electrophoresis and immunoblotting using the primary B/NS1-specific rabbit antiserum, mouse anti-PKR antibody 71/10 Ribogene or rabbit anti-PKR antibody Epitomics, mouse anti-NP antibody AbD Serotec, rabbit anti-phospho-PKR Thr446 antibody Cell Signaling, mouse anti.

  1. Phospho-PKR Thr446 Antibody detects endogenous levels of PKR only when phosphorylated at Thr446. Species Reactivity: Human, Monkey.
  2. Incubate membrane with Anti-rabbit IgG, HRP-linked Antibody 7074 at 1:2000 and anti-biotin, HRP-linked Antibody 7075 at 1:1000–1:3000 to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
  3. Invitrogen Anti-Phospho-PKR Thr446 Polyclonal, CatalogPA5-37704. Tested in Western Blot WB, Immunofluorescence IF, Immunocytochemistry ICC and Immunohistochemistry Paraffin IHC P applications. This antibody reacts with Human samples. Supplied as 100 µl purified antibody.

用于蛋白质翻译研究领域中研究 PKR Thr446/Thr451 磷酸盐的多克隆抗体。. 3076 Store at –20°C Phospho-PKR Thr446 Antibody rev. 02/09/07 n 100 µl 10 Western mini-blots W, IP H, Mk 74 kDa Rabbit Background: Protein kinase R PKR is transcriptionally induced by interferon and activated by double-stranded. RSK1, p70S6K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity 4,5. The Phospho-Ser/Thr Phe Antibody is a powerful tool for discovery of new proteins containing this important regulatory motif. Introduction. RNA-dependent protein kinase PKR is an independent prognostic variable in patients with non-small cell lung cancer NSCLC. In the present study, we investigated the correlation between PKR and 25 other biomarkers for NSCLC, identified the markers that could further improve the prognostic significance of PKR, and elucidated the mechanisms of interaction between these markers.

ERK Signaling Phospho Antibody Array The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample. Phospho-PKR Thr451 Antibody 3075 from Cell Signaling Technology. Polyclonal. Tested Applications: WB. Tested Reactivity: Human. Target Synonyms: anti-EIF2AK1, anti-eukaryotic translation initiation factor 2-alpha kinase 2, anti-interferon-inducible elF2alpha kinase, anti-PRKR, anti-P1/eIF-2A protein kinase, anti-interferon-induced, double-stranded RNA-activated protein kinase, anti-protein. Tyrosine Kinase Adaptor Phospho Antibody Array The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

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